High-throughput microfluidic micropipette aspiration device to probe time-scale dependent nuclear mechanics in intact cells.

The mechanical properties of the cell nucleus are more and more acknowledged as essential in many organic processes. The deformability of the nucleus determines the power of immune and most cancers cells to migrate via tissues and throughout endothelial cell layers, and modifications to the mechanical properties of the nucleus can function novel biomarkers in processes akin to most cancers development and stem cell differentiation.

However, present strategies to measure the viscoelastic nuclear mechanical properties are sometimes time consuming, restricted to probing one cell at a time, or require costly, extremely specialised gear. Furthermore, many present assays don’t measure time-dependent properties, that are attribute of viscoelastic supplies.

Here, we current an easy-to-use microfluidic device that applies the well-established method of micropipette aspiration, tailored to measure many cells in parallel. The device design permits speedy loading and purging of cells for measurements, and minimizes clogging by giant particles or clusters of cells. Combined with a semi-automated picture evaluation pipeline, the microfluidic device method allows considerably elevated experimental throughput.

We validated the experimental platform by evaluating computational fashions of the fluid mechanics in the device with experimental measurements of fluid circulation. In addition, we carried out experiments on cells missing the nuclear envelope protein lamin A/C and wild-type controls, which have well-characterized nuclear mechanical properties.

Fitting time-dependent nuclear deformation knowledge to energy legislation and totally different viscoelastic fashions revealed that lack of lamin A/C considerably altered the elastic and viscous properties of the nucleus, ensuing in considerably elevated nuclear deformability. Lastly, to reveal the flexibility of the units, we characterised the viscoelastic nuclear mechanical properties in a wide range of cell strains and experimental mannequin techniques, together with human pores and skin fibroblasts from a person with a mutation in the lamin gene related to dilated cardiomyopathy, wholesome management fibroblasts, induced pluripotent stem cells (iPSCs), and human tumor cells.

Taken collectively, these experiments reveal the power of the microfluidic device and automatic picture evaluation platform to present sturdy, excessive throughput measurements of nuclear mechanical properties, together with time-dependent elastic and viscous conduct, in a broad vary of functions.

Immobilization of Carbonic Anhydrase in Glass Micropipettes and Glass Fiber Filters for Flow-Through Reactor Applications.

There are varied methods of immobilizing carbonic anhydrase (CA) on stable supplies. One of the ultimate goals is to apply immobilized CA for the catalytic hydration of carbon dioxide (CO₂) as a primary step in the conversion of gaseous CO₂ into stable merchandise. The immobilization technique investigated permits a simple, secure, and quantifiable immobilization of bovine erythrocyte carbonic anhydrase (BCA) on silicate surfaces.

The technique is predicated on the usage of a water-soluble, polycationic second-generation dendronized polymer with on common 1000 repeating models, abbreviated as de-PG2₁₀₀₀. Several copies of BCA had been first covalently linked to de-PG2₁₀₀₀ via secure bisaryl hydrazone (BAH) bonds. Then, the de-PG2₁₀₀₀-BAH-BCA conjugates obtained had been adsorbed noncovalently both on microscopy glass coverslips, inside glass micropipettes, or in porous glass fiber filters.

The obvious density of the immobilized BCA on the glass surfaces was about 8-10 pmol/cm². In all three instances, the immobilized enzyme was extremely energetic and secure when examined with p-nitrophenyl acetate as a mannequin enzyme substrate at room temperature.

Fixvo II Micropipette - 500 Capacity -
LA607-1NO	EWC Diagnostics	1 unit	EUR 34.73
Description: Fixvo II Micropipette - 500 Capacity -

Varivol II Micropipette-1000 Capacity
LA614-1NO	EWC Diagnostics	1 unit	EUR 61.99
Description: Varivol II Micropipette-1000 Capacity

Varivol II Micropipette-1000 Capacity
LA616-1NO	EWC Diagnostics	1 unit	EUR 61.99
Description: Varivol II Micropipette-1000 Capacity

Varivol II Micropipette-5000 Capacity
LA619-1NO	EWC Diagnostics	1 unit	EUR 61.99
Description: Varivol II Micropipette-5000 Capacity

Varivol II Micropipette-200 Capacity :
LA613-1NO	EWC Diagnostics	1 unit	EUR 61.99
Description: Varivol II Micropipette-200 Capacity :

Varivol II Micropipette-100 Capacity :
LA615-1NO	EWC Diagnostics	1 unit	EUR 61.99
Description: Varivol II Micropipette-100 Capacity :

Varivol II Micropipette-200 Capacity :
LA618-1NO	EWC Diagnostics	1 unit	EUR 61.99
Description: Varivol II Micropipette-200 Capacity :

Varivol II Micropipette-10000 Capacity :
LA878-1NO	EWC Diagnostics	1 unit	EUR 75.54
Description: Varivol II Micropipette-10000 Capacity :

Varivol II Micropipette-10 Capacity :
LA611-1NO	EWC Diagnostics	1 unit	EUR 61.99
Description: Varivol II Micropipette-10 Capacity :

Varivol II Micropipette-50 Capacity :
LA612-1NO	EWC Diagnostics	1 unit	EUR 61.99
Description: Varivol II Micropipette-50 Capacity :

Varivol II Micropipette-20 Capacity :
LA617-1NO	EWC Diagnostics	1 unit	EUR 61.99
Description: Varivol II Micropipette-20 Capacity :

Fixvo II Micropipette - 5 Capacity - 5
LA599-1NO	EWC Diagnostics	1 unit	EUR 34.73
Description: Fixvo II Micropipette - 5 Capacity - 5

Fixvo II Micropipette - 10 Capacity -
LA600-1NO	EWC Diagnostics	1 unit	EUR 34.73
Description: Fixvo II Micropipette - 10 Capacity -

Micropipette Acura 825 0.1-2 uL Socorex - EACH
DD61104	Scientific Laboratory Supplies	EACH	EUR 488.7

Micropipette Acura 825 2-20 uL Socorex - EACH
DD61107	Scientific Laboratory Supplies	EACH	EUR 450.9

Micropipette Acura 825 20-200 uL Socorex - EACH
DD61110	Scientific Laboratory Supplies	EACH	EUR 450.9

Micropipette Acura 825 100-1000 uL Socorex - EACH
DD61111	Scientific Laboratory Supplies	EACH	EUR 450.9

Micropipette Acura 835 1-10 uL Socorex - EACH
DD61113	Scientific Laboratory Supplies	EACH	EUR 487.35

Pe Filter For Micropipette Protection - PK100
DD061968	Scientific Laboratory Supplies	PK100	EUR 152.55

µPet Autoclavable Micropipette Capacity:
LA955-1NO	EWC Diagnostics	1 unit	EUR 145.85
Description: µPet Autoclavable Micropipette Capacity:

µPet Autoclavable Micropipette Capacity:
LA956-1NO	EWC Diagnostics	1 unit	EUR 145.85
Description: µPet Autoclavable Micropipette Capacity:

µPet Autoclavable Micropipette Capacity:
LA957-1NO	EWC Diagnostics	1 unit	EUR 145.85
Description: µPet Autoclavable Micropipette Capacity:

µPet Autoclavable Micropipette Capacity:
LA958-1NO	EWC Diagnostics	1 unit	EUR 145.85
Description: µPet Autoclavable Micropipette Capacity:

µPet Autoclavable Micropipette Capacity:
LA959-1NO	EWC Diagnostics	1 unit	EUR 145.85
Description: µPet Autoclavable Micropipette Capacity:

µPet Autoclavable Micropipette Capacity:
LA960-1NO	EWC Diagnostics	1 unit	EUR 145.85
Description: µPet Autoclavable Micropipette Capacity:

µPet Autoclavable Micropipette Capacity:
LA961-1NO	EWC Diagnostics	1 unit	EUR 145.85
Description: µPet Autoclavable Micropipette Capacity:

µPet Autoclavable Micropipette Capacity:
LA962-1NO	EWC Diagnostics	1 unit	EUR 145.85
Description: µPet Autoclavable Micropipette Capacity:

µPet Autoclavable Micropipette Capacity:
LA963-1NO	EWC Diagnostics	1 unit	EUR 84.57
Description: µPet Autoclavable Micropipette Capacity:

µPet Autoclavable Micropipette 8-channel
LA964-1NO	EWC Diagnostics	1 unit	EUR 601.15
Description: µPet Autoclavable Micropipette 8-channel

µPet Autoclavable Micropipette 8-channel
LA965-1NO	EWC Diagnostics	1 unit	EUR 601.15
Description: µPet Autoclavable Micropipette 8-channel

µPet Autoclavable Micropipette Capacity:
LA966-1NO	EWC Diagnostics	1 unit	EUR 601.15
Description: µPet Autoclavable Micropipette Capacity:

µPet Autoclavable Micropipette 8-channel
LA967-1NO	EWC Diagnostics	1 unit	EUR 601.15
Description: µPet Autoclavable Micropipette 8-channel

Capillary micropipette Microcaps Drummond 0.5ulger) for pipettes 1 to 100 mL - PK100
DD075071	Scientific Laboratory Supplies	PK100	EUR 49.95

Positive displacement micropipette Acura capillar 846 volume 1 - 2 - 4 - 5 uL - EACH
DD61945	Scientific Laboratory Supplies	EACH	EUR 537.3

Positive displacement micropipette Acura capillar 846 volume 10 - 20 - 30 - 40 - 50 uL - EACH
DD61947	Scientific Laboratory Supplies	EACH	EUR 537.3

Positive displacement micropipette Acura capillar 846 volume 60 - 75 - 80 - 90 - 100 uL - EACH
DD61948	Scientific Laboratory Supplies	EACH	EUR 537.3

Controller Micro Pipette - EACH
PIP6110	Scientific Laboratory Supplies	EACH	EUR 109.35

0.5-10UL MAXYM ULTRA MICRO PIPET TIPS FOR PIPETMAN
T-300-L-STK	CORNING	480/pk	EUR 168
Description: Non Filter Tips; Maxymum Recovery Pipette Tips - Axygen

Holder f/ ELISA microplate&skirted PCR microplate, accessory for AgileMixer?
ACT-AVM3000-4	ACTGene	each	EUR 121.2

Microscope Slides, Micropure
22245-1	Polysciences Europe GmbH	1box	EUR 32

Microscope Slides, Micropure
22245-20	Polysciences Europe GmbH	1box	EUR 380

TAPE, MICROPORE, 1 IN
T969	PhytoTechnology Laboratories	120EA	EUR 5.57

Recombinant Boophilus microplus Antimicrobial peptide microplusin
MBS1357035-002mgBaculovirus	MyBiosource	0.02mg(Baculovirus)	EUR 1015

Recombinant Boophilus microplus Antimicrobial peptide microplusin
MBS1357035-002mgEColi	MyBiosource	0.02mg(E-Coli)	EUR 585

Recombinant Boophilus microplus Antimicrobial peptide microplusin
MBS1357035-002mgYeast	MyBiosource	0.02mg(Yeast)	EUR 765

Recombinant Boophilus microplus Antimicrobial peptide microplusin
MBS1357035-01mgEColi	MyBiosource	0.1mg(E-Coli)	EUR 695

Recombinant Boophilus microplus Antimicrobial peptide microplusin
MBS1357035-01mgYeast	MyBiosource	0.1mg(Yeast)	EUR 895

Micropeptin 478A
T25810-10mg	TargetMol Chemicals	10mg	Ask for price
Description: Micropeptin 478A

Micropeptin 478A
T25810-1g	TargetMol Chemicals	1g	Ask for price
Description: Micropeptin 478A

Micropeptin 478A
T25810-1mg	TargetMol Chemicals	1mg	Ask for price
Description: Micropeptin 478A

Micropeptin 478A
T25810-50mg	TargetMol Chemicals	50mg	Ask for price
Description: Micropeptin 478A

Micropeptin 478A
T25810-5mg	TargetMol Chemicals	5mg	Ask for price
Description: Micropeptin 478A

Micropeptin 478B
T25811-10mg	TargetMol Chemicals	10mg	Ask for price
Description: Micropeptin 478B

Micropeptin 478B
T25811-1g	TargetMol Chemicals	1g	Ask for price
Description: Micropeptin 478B

Micropeptin 478B
T25811-1mg	TargetMol Chemicals	1mg	Ask for price
Description: Micropeptin 478B

Micropeptin 478B
T25811-50mg	TargetMol Chemicals	50mg	Ask for price
Description: Micropeptin 478B

Micropeptin 478B
T25811-5mg	TargetMol Chemicals	5mg	Ask for price
Description: Micropeptin 478B

Micropeptin 478A
MBS5775541-5mg	MyBiosource	5(mg	EUR 915

Micropeptin 478A
MBS5775541-5x5mg	MyBiosource	5x5(mg	EUR 3970

Micropeptin 478B
MBS5775542-5mg	MyBiosource	5(mg	EUR 915

Micropeptin 478B
MBS5775542-5x5mg	MyBiosource	5x5(mg	EUR 3970

Micropore Tape - PK24
4702	Scientific Laboratory Supplies	PK24	EUR 17.6

MICROPESTILES
IB47315	IBI Scientific	50/PACK	EUR 34.15

48 WELL PCR MICROPLATE
PCR-48-C	CORNING	10/pk	EUR 122.4
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen

Micropestles 50 PCS
MP050	Geneaid Biotech	each	EUR 9.8

Microphyllone
TBZ0623	ChemNorm	unit	Ask for price

Microplate Sealer
abx292001-100Units	Abbexa	100 Units	EUR 75.6

Micro-Plate Washer
abx700010-1Unit	Abbexa	1 Unit	EUR 5258.4

Rack for up to 3 microplates
BV1010-MP	Benchmark Scientific	1 PC	EUR 171.67

Microplate Tray - EACH
MIX1368	Scientific Laboratory Supplies	EACH	EUR 27

Microplate Sealer
abx292001-100g	Abbexa	100 µg	Ask for price

Microplate Sealer
abx292001-20g	Abbexa	20 µg	EUR 50

Microplate Sealer
abx292001-50g	Abbexa	50 µg	Ask for price

KRV ELISA Microplate
MBS412136-48AntigenWells48ControlAntigenWells	MyBiosource	48AntigenWells&48ControlAntigenWells	EUR 275

KRV ELISA Microplate
MBS412136-5x48AntigenWells5x48ControlAntigenWells	MyBiosource	5x48AntigenWells&5x48ControlAntigenWells	EUR 1080

E. cun ELISA Microplate
MBS412158-48AntigenWells48ControlAntigenWells	MyBiosource	48AntigenWells&48ControlAntigenWells	EUR 275

E. cun ELISA Microplate
MBS412158-5x48AntigenWells5x48ControlAntigenWells	MyBiosource	5x48AntigenWells&5x48ControlAntigenWells	EUR 1080

SV5 ELISA Microplate
MBS412164-48AntigenWells48ControlAntigenWells	MyBiosource	48AntigenWells&48ControlAntigenWells	EUR 275

SV5 ELISA Microplate
MBS412164-5x48AntigenWells5x48ControlAntigenWells	MyBiosource	5x48AntigenWells&5x48ControlAntigenWells	EUR 1080

Microprobes and Picks
07397-1	Polysciences Europe GmbH	1set	EUR 114

ATP Microplate Assay Kit
DLSM0119	DL Develop	100 Assays	EUR 385
Description: Detection and Quantification of ATP Content.

96-well microplates
EA-004	Creative BioMart	each	EUR 263.2

3M Micropore Tape 2.50cm x 9.1m - PK12
MM1530-250	Scientific Laboratory Supplies	PK12	EUR 11.87

Ro I (Romalea microptera)
047-72	PHOENIX PEPTIDE	1 mg	EUR 177.12

Ro II (Romalea microptera)
047-73	PHOENIX PEPTIDE	500 μg	EUR 73.44

ATP Microplate Assay Kit
MBS8309599-100Assays	MyBiosource	100Assays	EUR 440

ATP Microplate Assay Kit
MBS8309599-5x100Assays	MyBiosource	5x100Assays	EUR 1920

TMEV ELISA Microplate
MBS412113-48AntigenWells48ControlAntigenWells	MyBiosource	48AntigenWells&48ControlAntigenWells	EUR 275

TMEV ELISA Microplate
MBS412113-5x48AntigenWells5x48ControlAntigenWells	MyBiosource	5x48AntigenWells&5x48ControlAntigenWells	EUR 1080

LCMV ELISA Microplate
MBS412122-48AntigenWells48ControlAntigenWells	MyBiosource	48AntigenWells&48ControlAntigenWells	EUR 275

LCMV ELISA Microplate
MBS412122-5x48AntigenWells5x48ControlAntigenWells	MyBiosource	5x48AntigenWells&5x48ControlAntigenWells	EUR 1080

RCV/SDA ELISA Microplate
MBS412127-48AntigenWells48ControlAntigenWells	MyBiosource	48AntigenWells&48ControlAntigenWells	EUR 275

RCV/SDA ELISA Microplate
MBS412127-5x48AntigenWells5x48ControlAntigenWells	MyBiosource	5x48AntigenWells&5x48ControlAntigenWells	EUR 1080

EDIM ELISA Microplate
MBS412144-48AntigenWells48ControlAntigenWells	MyBiosource	48AntigenWells&48ControlAntigenWells	EUR 275

EDIM ELISA Microplate
MBS412144-5x48AntigenWells5x48ControlAntigenWells	MyBiosource	5x48AntigenWells&5x48ControlAntigenWells	EUR 1080

CARB ELISA Microplate
MBS412147-48AntigenWells48ControlAntigenWells	MyBiosource	48AntigenWells&48ControlAntigenWells	EUR 275

CARB ELISA Microplate
MBS412147-5x48AntigenWells5x48ControlAntigenWells	MyBiosource	5x48AntigenWells&5x48ControlAntigenWells	EUR 1080

ATP Microplate Assay Kit
RDSM119	Reddot Biotech	100 Assays	EUR 385
Description: Detection and Quantification of ATP Content.

QuickSeal Micro Microplate film 135mm x 80mm - PK100
MIC9220	Scientific Laboratory Supplies	PK100	EUR 62.1

Linearity LQ Microalbumin/Microprotein
K722M-5	Audit MicroControls	5 x 2 mL	EUR 374

Iron Microplate Assay Kit
DLSM0106	DL Develop	100 Assays	EUR 315
Description: Detection and Quantification of Ferrium Content.

Zinc Microplate Assay Kit
DLSM0110	DL Develop	100 Assays	EUR 525
Description: Detection and Quantification of Zinc Content.

Urea Microplate Assay Kit
DLSM0147	DL Develop	100 Assays	EUR 175
Description: Detection and Quantification of Urea Content.

Heme Microplate Assay Kit
DLSM0151	DL Develop	100 Assays	EUR 210
Description: Detection and Quantification of Heme Content.

Horn microplate only - EACH
HOM2618	Scientific Laboratory Supplies	EACH	EUR 5622.57

24 WELL PCR MICROPLATE. CLEAR
PCR-24-C	CORNING	10/pk	EUR 87.6
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen

32 WELL PCR MICROPLATE. CLEAR
PCR-32-C	CORNING	10/pk	EUR 105.6
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen

96 WELL CLEAR PCR MICROPLATE
PCR-96-C	CORNING	10/pk	EUR 174
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

Rack for up to 3 microplates - EACH
MIX7040	Scientific Laboratory Supplies	EACH	EUR 252.45

Urea Microplate Assay Kit
MBS8305389-100Assays	MyBiosource	100Assays	EUR 330

Urea Microplate Assay Kit
MBS8305389-5x100Assays	MyBiosource	5x100Assays	EUR 1420

Heme Microplate Assay Kit
MBS8305393-100Assays	MyBiosource	100Assays	EUR 350

Heme Microplate Assay Kit
MBS8305393-5x100Assays	MyBiosource	5x100Assays	EUR 1510

K Virus ELISA Microplate
MBS412134-48AntigenWells48ControlAntigenWells	MyBiosource	48AntigenWells&48ControlAntigenWells	EUR 340

K Virus ELISA Microplate
MBS412134-5x48AntigenWells5x48ControlAntigenWells	MyBiosource	5x48AntigenWells&5x48ControlAntigenWells	EUR 1385

Iron Microplate Assay Kit
RDSM106	Reddot Biotech	100 Assays	EUR 315
Description: Detection and Quantification of Ferrium Content.

Zinc Microplate Assay Kit
RDSM110	Reddot Biotech	100 Assays	EUR 525
Description: Detection and Quantification of Zinc Content.

Urea Microplate Assay Kit
RDSM147	Reddot Biotech	100 Assays	EUR 175
Description: Detection and Quantification of Urea Content.

Heme Microplate Assay Kit
RDSM151	Reddot Biotech	100 Assays	EUR 210
Description: Detection and Quantification of Heme Content.

Urea Microplate Assay Kit
ASK1160	Bioworld Biotech	100 Assays	EUR 198
Description: Detection and Quantification of Urea Content.

Heme Microplate Assay Kit
ASK1164	Bioworld Biotech	100 Assays	EUR 228
Description: Detection and Quantification of Heme Content.

NAD/NADH Microplate Assay Kit
DLSM0008	DL Develop	100 Assays	EUR 385
Description: Detection and Quantification of NAD/NADH Content.

Thiol Microplate Assay Kit
DLSM0146	DL Develop	100 Assays	EUR 245
Description: Detection and Quantification of Thiol Content.

TBARS Microplate Assay Kit
DLSM0148	DL Develop	100 Assays	EUR 210
Description: Detection and Quantification of Thiobarbituric Acid Reactive Substances (TBARS) Content.

384 WELL PCR MICROPLATE. CLEAR
PCR-384-C	CORNING	10/pk	EUR 219.6
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

Microplate Deep 96 Well Pp 2.0ml - PK25
P2MLSQC	Scientific Laboratory Supplies	PK25	EUR 148.5

Thiol Microplate Assay Kit
MBS8305388-100Assays	MyBiosource	100Assays	EUR 365

Thiol Microplate Assay Kit
MBS8305388-5x100Assays	MyBiosource	5x100Assays	EUR 1580

TBARS Microplate Assay Kit
MBS8305390-100Assays	MyBiosource	100Assays	EUR 350

TBARS Microplate Assay Kit
MBS8305390-5x100Assays	MyBiosource	5x100Assays	EUR 1510

Mouse RSV ELISA Microplate
MBS412038-48AntigenWells48ControlAntigenWells	MyBiosource	48AntigenWells&48ControlAntigenWells	EUR 275

Mouse RSV ELISA Microplate
MBS412038-5x48AntigenWells5x48ControlAntigenWells	MyBiosource	5x48AntigenWells&5x48ControlAntigenWells	EUR 1080

Mouse CMV ELISA Microplate
MBS412157-48AntigenWells48ControlAntigenWells	MyBiosource	48AntigenWells&48ControlAntigenWells	EUR 275

Mouse CMV ELISA Microplate
MBS412157-5x48AntigenWells5x48ControlAntigenWells	MyBiosource	5x48AntigenWells&5x48ControlAntigenWells	EUR 1080

NAD/NADH Microplate Assay Kit
RDSM008	Reddot Biotech	100 Assays	EUR 385
Description: Detection and Quantification of NAD/NADH Content.

Thiol Microplate Assay Kit
RDSM146	Reddot Biotech	100 Assays	EUR 245
Description: Detection and Quantification of Thiol Content.

TBARS Microplate Assay Kit
RDSM148	Reddot Biotech	100 Assays	EUR 210
Description: Detection and Quantification of Thiobarbituric Acid Reactive Substances (TBARS) Content.

NAD/NADH Microplate Assay Kit
ASK1008	Bioworld Biotech	100 Assays	EUR 398
Description: Detection and Quantification of NAD/NADH Content.

Thiol Microplate Assay Kit
ASK1159	Bioworld Biotech	100 Assays	EUR 268
Description: Detection and Quantification of Thiol Content.

TBARS Microplate Assay Kit
ASK1161	Bioworld Biotech	100 Assays	EUR 228
Description: Detection and Quantification of Thiobarbituric Acid Reactive Substances (TBARS) Content.

0.5-10UL PRESTERILIZED ULTRA MICRO PIPET TIPS FOR PIPETMAN
T-300-STK-S	CORNING	480/pk	EUR 168
Description: Non Filter Tips; Pipette Tips - Axygen

AGAR, MICROPROPAGATION
A111	PhytoTechnology Laboratories	100G	EUR 4.37

Na+/K+ ATPase Microplate Assay Kit
DLSM0019	DL Develop	100 Assays	EUR 315
Description: Detection and Quantification of Na+/K+ ATPase Activity.

Starch Microplate Assay Kit
DLSM0022	DL Develop	100 Assays	EUR 350
Description: Detection and Quantification of Starch Content.

Uric Acid Microplate Assay Kit
DLSM0058	DL Develop	100 Assays	EUR 385
Description: Detection and Quantification of Uric Acid Content.

Tannin Microplate Assay Kit
DLSM0060	DL Develop	100 Assays	EUR 402.5
Description: Detection and Quantification of Tannin Content.

Pepsin Microplate Assay Kit
DLSM0081	DL Develop	100 Assays	EUR 315
Description: Detection and Quantification of Pepsin Activity.

Lipase Microplate Assay Kit
DLSM0083	DL Develop	100 Assays	EUR 385
Description: Detection and Quantification of Lipase Activity.

Kalium Microplate Assay Kit
DLSM0104	DL Develop	100 Assays	EUR 280
Description: Detection and Quantification of Kalium Content.

Copper Microplate Assay Kit
DLSM0143	DL Develop	100 Assays	EUR 315
Description: Detection and Quantification of Copper Content.

Urease Microplate Assay Kit
DLSM0166	DL Develop	100 Assays	EUR 420
Description: Detection and Quantification of Urease Activity.

Lysine Microplate Assay Kit
DLSM0187	DL Develop	100 Assays	EUR 420
Description: Detection and Quantification of Lysine Content.

Pectin Microplate Assay Kit
DLSM0204	DL Develop	100 Assays	EUR 350
Description: Detection and Quantification of Pectin Content.

AGAR, MICROPROPAGATION
C968	PhytoTechnology Laboratories	1EA	EUR 53.47

Microplate vessel holder
LBI10231	Westburg	each	EUR 385.32

Microplate Lid N Ster w/o rings - PK50
PCR0390	Scientific Laboratory Supplies	PK50	EUR 152.55

Lipase Microplate Assay Kit
MBS8243187-100Assays	MyBiosource	100Assays	EUR 440

Lipase Microplate Assay Kit
MBS8243187-5x100Assays	MyBiosource	5x100Assays	EUR 1920

Tannin Microplate Assay Kit
MBS8243197-100Assays	MyBiosource	100Assays	EUR 445

Tannin Microplate Assay Kit
MBS8243197-5x100Assays	MyBiosource	5x100Assays	EUR 1955

Starch Microplate Assay Kit
MBS8243225-100Assays	MyBiosource	100Assays	EUR 420

Starch Microplate Assay Kit
MBS8243225-5x100Assays	MyBiosource	5x100Assays	EUR 1830

Na+/K+ ATPase Microplate Assay Kit
MBS8243226-100Assays	MyBiosource	100Assays	EUR 400

Na+/K+ ATPase Microplate Assay Kit
MBS8243226-5x100Assays	MyBiosource	5x100Assays	EUR 1735

Uric Acid Microplate Assay Kit
MBS8243236-100Assays	MyBiosource	100Assays	EUR 440

Uric Acid Microplate Assay Kit
MBS8243236-5x100Assays	MyBiosource	5x100Assays	EUR 1920

Pepsin Microplate Assay Kit
MBS8243252-100Assays	MyBiosource	100Assays	EUR 400

Pepsin Microplate Assay Kit
MBS8243252-5x100Assays	MyBiosource	5x100Assays	EUR 1735

Lysine Microplate Assay Kit
MBS8309625-100Assays	MyBiosource	100Assays	EUR 455

Lysine Microplate Assay Kit
MBS8309625-5x100Assays	MyBiosource	5x100Assays	EUR 1985

Pectin Microplate Assay Kit
MBS8309633-100Assays	MyBiosource	100Assays	EUR 420

Pectin Microplate Assay Kit
MBS8309633-5x100Assays	MyBiosource	5x100Assays	EUR 1830

Urease Microplate Assay Kit
MBS8305401-100Assays	MyBiosource	100Assays	EUR 455

Urease Microplate Assay Kit
MBS8305401-5x100Assays	MyBiosource	5x100Assays	EUR 1985

Copper Microplate Assay Kit
MBS8292800-100Assays	MyBiosource	100Assays	EUR 400

Copper Microplate Assay Kit
MBS8292800-5x100Assays	MyBiosource	5x100Assays	EUR 1735

Mouse LDHV ELISA Microplate
MBS412033-48AntigenWells48ControlAntigenWells	MyBiosource	48AntigenWells&48ControlAntigenWells	EUR 340

Mouse LDHV ELISA Microplate
MBS412033-5x48AntigenWells5x48ControlAntigenWells	MyBiosource	5x48AntigenWells&5x48ControlAntigenWells	EUR 1385

Rabbit Pox ELISA Microplate
MBS412054-48AntigenWells48ControlAntigenWells	MyBiosource	48AntigenWells&48ControlAntigenWells	EUR 275

The micropipettes and the glass fiber filters had been utilized as flow-through techniques for steady operation at room temperature. In the case of the glass fiber filters, the filters had been positioned inside a selfmade flow-through filter holder which permits flow-through runs with a couple of filter linked in sequence. This affords the chance of accelerating the substrate conversion by rising the variety of BCA-containing filters.