Mechanical properties of bovine erythrocytes derived from ion current measurements using micropipettes.

Synchronized information from ion currents and optical microscopy have been used to measure the mechanical properties of particular person mushy microparticles. This new experimental technique attracts on the indicators generated by every particle because it passes by means of the tip of a pipette.

The method represents an advance on micropipette aspiration (MA), which makes use of optical microscopy in isolation. The ionic resistance by means of the tip supplies further data relating to the progress of particle deformation. The augmented method is demonstrated by learning aspiration of particular person bovine erythrocytes right into a micropipette.

Effective mechanical properties have been deduced by using the ionic current response to measure the dwell time and velocity of erythrocytes. Values obtained for the efficient viscosity signify liquid-like deformation of the entire cell, and present a constant shear thinning impact. The efficient elasticity additionally varies with utilized pressure, and was greater for erythrocytes saved for a number of weeks.

The information present that wall friction is a crucial issue for the uptake dynamics, and that the erythrocytes have been broken or ruptured when passing by means of pipettes of opening diameter 0.7 μm, the smallest used. These outcomes exhibit a brand new method to measuring the mechanics of particular person bioparticles.

The biomechanical properties of single cells present nice potential for early illness analysis and efficient therapies. In this examine, a microfluidic machine was developed for quantifying the mechanical properties of a single cell. Micropipette aspiration was built-in right into a microfluidic machine that mimics a classical Wheatstone bridge circuit.

This method permits us not solely to successfully alter the move route for single-cell trapping, but additionally to exactly management the stress exerted on the aspirated cells, analogous to the characteristic of the Wheatstone bridge that may exactly management bridge voltage and current. By combining the micropipette aspiration method into the microfluidic machine, we are able to successfully entice the microparticles and Hela cells in addition to measure the deformability of cells.

The Young’s modulus of Hela cells was evaluated to be 387 ± 77 Pa, which is according to earlier micropipette aspiration research. The simplicity, precision, and usefulness of our machine present good potential for biomechanical trials in scientific analysis and cell biology analysis.

Micro-Surface and -Interfacial Tensions Measured Using the Micropipette Technique: Applications in Ultrasound-Microbubbles, Oil-Recovery, Lung-Surfactants, Nanoprecipitation, and Microfluidics.

This assessment presents a sequence of measurements of the floor and interfacial tensions we’ve been in a position to make using the micropipette method. These embody: equilibrium tensions on the air-water floor and oil-water interface, in addition to equilibrium and dynamic adsorption of water-soluble surfactants and water-insoluble and lipids. At its essence, the micropipette method is one of capillary-action, glass-wetting, and utilized stress.

A micropipette, as a parallel or tapered shaft, is mounted horizontally in a microchamber and considered in an inverted microscope. When full of air or oil, and inserted into an aqueous-filled chamber, the place of the floor or interface meniscus is managed by utilized micropipette stress.

The place and therefore radius of curvature of the meniscus will be moved in a managed vogue from dimensions related to the capillary tip (~5⁻10 μm), to again down the micropipette that may taper out to 450 μm. All measurements are due to this fact truly made on the microscale. Following the Young⁻Laplace equation and geometry of the capillary, the floor or interfacial rigidity worth is just obtained from the radius of the meniscus within the tapered pipette and the utilized stress to maintain it there.

Motivated by Franklin’s early experiments that demonstrated molecularity and monolayer formation, we additionally give a short potted-historical perspective that features basic surfactancy pushed by margarine, the primary use of a micropipette to circuitously measure bilayer membrane tensions and free energies of formation, and its foundation for revolutionising the examine and functions of membrane ion-channels in Droplet Interface Bilayers.

Finally, we give 5 examples of the place our measurements have had an impression on functions in micro-surfaces and microfluidics, together with fuel microbubbles for ultrasound distinction; interfacial tensions for micro-oil droplets in oil restoration; floor tensions and tensions-in-the floor for pure and artificial lung surfactants; interfacial rigidity in nanoprecipitation; and micro-surface tensions in microfluidics.

Micropipette pressure sensors for in vivo pressure measurements on single cells and multicellular microorganisms.

Measuring forces from the piconewton to millinewton vary is of nice significance for the examine of dwelling methods from a biophysical perspective. The use of versatile micropipettes as extremely delicate pressure probes has turn out to be established within the biophysical group, advancing our understanding of mobile processes and microbial habits.

The micropipette pressure sensor (MFS) method depends on measurement of the forces appearing on a force-calibrated, hole glass micropipette by optically detecting its deflections. The MFS method covers a large micro- and mesoscopic regime of detectable forces (tens of piconewtons to millinewtons) and pattern sizes (micrometers to millimeters), doesn’t require gluing of the pattern to the cantilever, and permits simultaneous optical imaging of the pattern all through the experiment.

50µL Transfer Pipettes
TP50	BioAssay Systems	N/A	EUR 19

100µL Transfer Pipettes
TP100	BioAssay Systems	N/A	EUR 19

1000µL Transfer Pipettes
TP1000	BioAssay Systems	N/A	EUR 19

Pipettes Pyrex 50ml - PK50
TIS1608	Scientific Laboratory Supplies	PK50	EUR 324

Module for pipettes - EACH
WAS7232	Scientific Laboratory Supplies	EACH	EUR 1652.4

Carousel for 6 Pipettes
P4280-CR	MTC Bio	1/pack	EUR 98.45
Description: Carousel for 6 Pipettes

Pipettes Pyrex 10ml - PK400
TIS1604	Scientific Laboratory Supplies	PK400	EUR 891

Pipette mini stand for 3 pipettes
TH9420320	Westburg	each	EUR 76.85

Corning Pipettes - 5000ul - AP-5000 - EACH
AP-5000	Scientific Laboratory Supplies	EACH	EUR 330.75

Pipette stand round, for 6 pipettes
LHP-PS02	Phoenix instrument	each	EUR 64

Pipette stand linear, for 5 pipettes
LHP-PS01	Phoenix instrument	each	EUR 40

electrical Pipette filler for all glas- and plastic pipettes
LHP-PH01	Phoenix instrument	each	EUR 205

SureStand™ Pipette Stand for 6 Halo™ Single Channel Pipettes or 6 ProPette LE Single Channel Pipettes, up to four ProPette LE Multi-channel Pipettes
P4406-P	MTC Bio	1	EUR 94.8

Waste box for pipettes - PK16
DD30535	Scientific Laboratory Supplies	PK16	EUR 356.4

Finnpipette Pipette stand for 6 pipettes
TH9420290	Westburg	each	EUR 94.29

Carrousel For 7 Pipettes - EACH
F161401	Scientific Laboratory Supplies	EACH	EUR 124.2

Bulb Pipettes 10mL 1 mark AS
PIP12100	Scientific Laboratory Supplies	PK5	EUR 55.97

Bulb Pipettes 25mL 1 mark AS
PIP12160	Scientific Laboratory Supplies	PK5	EUR 67.26

Carousel for 6 Halo™ Pipettes
H6800-CR	MTC Bio	1	EUR 89.5

SteriWare Jumbo Pipettes 1100mm. Box of 37 - PK37
SAM1241	Scientific Laboratory Supplies	PK37	EUR 255.15

Aspirating Pipettes Sterile 2mL - PK500
ARG1022	Scientific Laboratory Supplies	PK500	EUR 496.8

Aspirating Pipettes Sterile 5mL - PK200
ARG1024	Scientific Laboratory Supplies	PK200	EUR 232.2

Corning 5ml Aspirating Pipettes - PK200
X14065	Scientific Laboratory Supplies	PK200	EUR 184.95

Open Ended Pipettes Gradd Sterile W filter 1mL - PK500
ARG1468	Scientific Laboratory Supplies	PK500	EUR 303.75

Open Ended Pipettes Gradd Sterile W filter 2mL - PK500
ARG1472	Scientific Laboratory Supplies	PK500	EUR 376.65

Open Ended Pipettes Gradd Sterile W filter 5mL - PK200
ARG1474	Scientific Laboratory Supplies	PK200	EUR 218.7

Aspirating Pipettes Sterile 10mL - PK200
ARG1020	Scientific Laboratory Supplies	PK200	EUR 213.3

Bulb Pipettes 2mL 1 mark AS - PK5
PIP12020	Scientific Laboratory Supplies	PK5	EUR 95.85

Bulb Pipettes 3mL 1 mark AS - PK5
PIP12040	Scientific Laboratory Supplies	PK5	EUR 55.35

Bulb Pipettes 4mL 1 mark AS - PK5
PIP12060	Scientific Laboratory Supplies	PK5	EUR 82.35

Bulb Pipettes 5mL 1 mark AS - PK5
PIP12080	Scientific Laboratory Supplies	PK5	EUR 108

Disposable Plastic Serological Pipettes 100ml - PK50
ARG1594	Scientific Laboratory Supplies	PK50	EUR 549.45

Open Ended Pipettes Gradd Sterile W filter 10mL - PK200
ARG1470	Scientific Laboratory Supplies	PK200	EUR 217.35

Bulb Pipettes 15mL 1 mark AS - PK5
PIP12120	Scientific Laboratory Supplies	PK5	EUR 97.2

Bulb Pipettes 20mL 1 mark AS - PK5
PIP12140	Scientific Laboratory Supplies	PK5	EUR 102.6

Serological Pipettes, 5ml, 400 per case
SP102116	Bio Basic	1CS, 400UNIT	EUR 215.93

SteriWare Jumbo Pipettes 450mm. Box of 50 - PK50
SAM1237	Scientific Laboratory Supplies	PK50	EUR 272.7

SteriWare Jumbo Pipettes 900mm. Box of 50 - PK50
SAM1239	Scientific Laboratory Supplies	PK50	EUR 344.25

Silicone Grease For Pipettes 45g - EACH
E0013019213	Scientific Laboratory Supplies	EACH	EUR 132.19

Serological Pipettes, 10ml, 400 per case
SP102121	Bio Basic	1CS, 400UNIT	EUR 189.13

Serological Pipettes, 25ml, 200 per case
SP102136	Bio Basic	1CS, 200UNIT	EUR 178.8

Serological Pipettes, 50ml, 160 per case
SP102161	Bio Basic	1CS, 160UNIT	EUR 364.32

Bulb Pipettes 100mL 1 mark AS + Certificate - PK5
PIP1250	Scientific Laboratory Supplies	PK5	EUR 135

Disposable Serological Pipettes 5ml - PK500
ARG1564	Scientific Laboratory Supplies	PK500	EUR 452.25

UNIVERSAL LINEAR RACK FOR SIX PIPETTES
4079	CORNING	1/pk	EUR 84
Description: Corning and Axygen Liquid Handling Equipment; Corning Lambda Plus Single Channel Pipettors

Rubber teat for pasteur pipettes - PK100
PIP1026	Scientific Laboratory Supplies	PK100	EUR 167.4

Disposable Serological Pipettes 1ml - PK1000
ARG1552	Scientific Laboratory Supplies	PK1000	EUR 519.75

Disposable Serological Pipettes 2ml - PK1000
ARG1558	Scientific Laboratory Supplies	PK1000	EUR 699.3

Disposable Serological Pipettes 25mL - PK150
ARG1562	Scientific Laboratory Supplies	PK150	EUR 372.6

Disposable Serological Pipettes 50mL - PK100
ARG1566	Scientific Laboratory Supplies	PK100	EUR 648

BRAND VOLUMETRIC PIPETTES KBCERTIFIED - PK12
Z325295-12EA	Scientific Laboratory Supplies	PK12	EUR 140.4

Serological Pipettes, 100ml , Sterile, Individually Wrapped
LC3067-050	GenDepot	50/Pk	EUR 465.6

UNIVERSAL CAROUSEL RACK FOR SIX PIPETTES
4078	CORNING	1/pk	EUR 158.4
Description: Corning and Axygen Liquid Handling Equipment; Corning Lambda Plus Single Channel Pipettors

Sterilisation box for Pasteur pipettes 230 mm - EACH
DD41301	Scientific Laboratory Supplies	EACH	EUR 117.45

SteriWare Jumbo Pipettes 1100mm. Sterile.. Box of 37 - PK37
SAM1242	Scientific Laboratory Supplies	PK37	EUR 287.55

Eppendorf Research Plus Pipette Option 1 pipettes - EACH
E3123000900	Scientific Laboratory Supplies	EACH	EUR 849.15

Eppendorf Research Plus Pipette Option 2 pipettes - EACH
E3123000918	Scientific Laboratory Supplies	EACH	EUR 795.15

Eppendorf Research Plus Pipette Option 3 pipettes - EACH
E3123000926	Scientific Laboratory Supplies	EACH	EUR 849.15

Trio Portable Rack For 3 Pipettes - EACH
F161405	Scientific Laboratory Supplies	EACH	EUR 110.7

5ml Tips For Gilson Pipettes Lse - PK2500
PIP0073	Scientific Laboratory Supplies	PK2500	EUR 502.2

BRAND VOLUMETRIC PIPETTES KB CERTFIEDC - PK6
Z325139-6EA	Scientific Laboratory Supplies	PK6	EUR 68.85

Pipettor Clamp-ette Triple Blue - EACH
PIP9427	Scientific Laboratory Supplies	EACH	EUR 108

Acrylic Pipette Rack for 1mL-100mL Pipettes Blue - EACH
ARG1026	Scientific Laboratory Supplies	EACH	EUR 390.15

Acrylic Pipette Rack for 1mL-100mL Pipettes lear - EACH
ARG1028	Scientific Laboratory Supplies	EACH	EUR 692.55

Mini Serological Pipettes 5mL PS 9 - PK200
ARG1582	Scientific Laboratory Supplies	PK200	EUR 218.7

Bulb Pipettes 10mL 1 mark AS + Certificate
PIP12400	Scientific Laboratory Supplies	PK5	EUR 78.66

Disposable Pipettes 0.01 Graduations 1ml - PK500
ARG1588	Scientific Laboratory Supplies	PK500	EUR 303.75

Mini Serological Pipettes 10mL PS 9 - PK150
ARG1578	Scientific Laboratory Supplies	PK150	EUR 187.65

Mini Serological Pipettes 25mL PS 9 - PK100
ARG1580	Scientific Laboratory Supplies	PK100	EUR 272.7

SteriWare Jumbo Pipettes 450mm. Sterile.. Box of 50 - PK50
SAM1238	Scientific Laboratory Supplies	PK50	EUR 295.65

SteriWare Jumbo Pipettes 900mm. Sterile.. Box of 50 - PK50
SAM1240	Scientific Laboratory Supplies	PK50	EUR 399.6

Biosafe Serological Pipettes Plastic 5ml - PK200
ARG1094	Scientific Laboratory Supplies	PK200	EUR 226.8

Biosafe Serological Pipettes Plastic 10ml - PK200
ARG1090	Scientific Laboratory Supplies	PK200	EUR 274.05

Biosafe Serological Pipettes Plastic 25ml - PK150
ARG1092	Scientific Laboratory Supplies	PK150	EUR 351

Disposable Serological Pipettes 1.1ml Milk - PK1000
ARG1584	Scientific Laboratory Supplies	PK1000	EUR 653.4

mLINE pipette multipack - 3 mLINE 20 pipettes (2-20 uL 20-200 uL 100-1000 uL)
DD725662	Scientific Laboratory Supplies	EACH	EUR 894.9

Disposable Serological Pipettes Multi. 2.2mL - PK1000
ARG1560	Scientific Laboratory Supplies	PK1000	EUR 699.3

Disposable Plastic Serological Pipettes 1ml - PK500
ARG1590	Scientific Laboratory Supplies	PK500	EUR 311.85

Disposable Plastic Serological Pipettes 2mL - PK500
ARG1598	Scientific Laboratory Supplies	PK500	EUR 378

Disposable Plastic Serological Pipettes 5ml - PK200
ARG1604	Scientific Laboratory Supplies	PK200	EUR 217.35

Bulb Pipettes 1mL 1 mark AS + Certificate - PK5
PIP12300	Scientific Laboratory Supplies	PK5	EUR 97.2

Bulb Pipettes 2mL 1 mark AS + Certificate - PK5
PIP12320	Scientific Laboratory Supplies	PK5	EUR 97.2

Bulb Pipettes 3mL 1 mark AS + Certificate - PK5
PIP12340	Scientific Laboratory Supplies	PK5	EUR 102.6

Bulb Pipettes 4mL 1 mark AS + Certificate - PK5
PIP12360	Scientific Laboratory Supplies	PK5	EUR 116.1

Bulb Pipettes 5mL 1 mark AS + Certificate - PK5
PIP12380	Scientific Laboratory Supplies	PK5	EUR 106.65

Disposable Plastic Serological Pipettes 10ml - PK200
ARG1592	Scientific Laboratory Supplies	PK200	EUR 234.9

Disposable Plastic Serological Pipettes 25mL - PK150
ARG1602	Scientific Laboratory Supplies	PK150	EUR 369.9

Disposable Plastic Serological Pipettes 50ml - PK100
ARG1606	Scientific Laboratory Supplies	PK100	EUR 684.45

Sterilisation box for Pasteur pipettes up to 170 mm - EACH
DD41300	Scientific Laboratory Supplies	EACH	EUR 114.75

Bulb Pipettes 20mL 1 mark AS + Certificate - PK5
PIP12440	Scientific Laboratory Supplies	PK5	EUR 135

Bulb Pipettes 25mL 1 mark AS + Certificate - PK5
PIP12460	Scientific Laboratory Supplies	PK5	EUR 136.35

Bulb Pipettes 50mL 1 mark AS + Certificate - PK5
PIP12480	Scientific Laboratory Supplies	PK5	EUR 171.45

Pipette Tips, Filtered, 300µL capacity, sterile, for 200µL and 300µL pipettes, 10 racks of 96 tips
P4300-FT	MTC Bio	960	EUR 106.8

HandEvac Rubber Adapter for Pasteur Pipettes - EACH
ARG1286	Scientific Laboratory Supplies	EACH	EUR 27

Serological Pipettes, 1ml , Sterile, Individually Wrapped
LC3061-500	GenDepot	500/pk	EUR 176.4

Serological Pipettes, 2ml , Sterile, Individually Wrapped
LC3062-400	GenDepot	400/pk	EUR 165.6

Serological Pipettes, 5ml , Sterile, Individually Wrapped
LC3063-200	GenDepot	200/pk	EUR 133.2

E1-Cliptip Charging Stand for 3 pipettes - EACH
PIP0570	Scientific Laboratory Supplies	EACH	EUR 1065.15

Serological Pipettes, 10ml , Sterile, Individually Wrapped
LC3064-200	GenDepot	200/pk	EUR 138

Serological Pipettes, 25ml , Sterile, Individually Wrapped
LC3065-200	GenDepot	200/pk	EUR 208.8

Serological Pipettes, 50ml , Sterile, Individually Wrapped
LC3066-100	GenDepot	100/pk	EUR 278.4

Disposable Serological Pipettes 10ml *Wide Tip* - PK400
ARG1556	Scientific Laboratory Supplies	PK400	EUR 384.75

Disposable Serological Pipettes *Wide Tip* 10mL - PK200
ARG1596	Scientific Laboratory Supplies	PK200	EUR 222.75

Pipettes 1ml 0.01 Graduations (40 packs of 25) - PK1000
ARG1550	Scientific Laboratory Supplies	PK1000	EUR 519.75

Disposable Plastic Serological Pipettes Milk 1.1ml - PK500
ARG1586	Scientific Laboratory Supplies	PK500	EUR 367.2

Reference 2 Pack 1 0.5-10; 10-100; 100-1000uL pipettes
E4920000903	Scientific Laboratory Supplies	EACH	EUR 835.62

Reference 2 Pack 2 2-20; 20-200; 100-1000uL pipettes
E4920000911	Scientific Laboratory Supplies	EACH	EUR 835.62

set of 4 genesig easy pipettes, 5ul, 10ul, 200ul & 500ul
Z-genesigEASY-Pipettes	Novacyt Group	4 pipettes	EUR 329

Oxford Serological Pipettes, 100mL, Pink, Sterile, Individually Wrapped (50pcs)
OLOSP-100C	Westburg	each	EUR 100.28

Mouse Actin Filament Associated Protein 1 (AFAP1) Antibody Pair Kit (with Standard)
MBS2101506-10x96Tests	MyBiosource	10x96Tests	EUR 1515

Mouse Actin Filament Associated Protein 1 (AFAP1) Antibody Pair Kit (with Standard)
MBS2101506-10x96TestsMBS2090685AbPairsSupportPack110x96Tests	MyBiosource	10x96Tests+MBS2090685(AbPairsSupportPack1,10x96Tests)	EUR 1640

Mouse Actin Filament Associated Protein 1 (AFAP1) Antibody Pair Kit (with Standard)
MBS2101506-5x96Tests	MyBiosource	5x96Tests	EUR 960

Mouse Actin Filament Associated Protein 1 (AFAP1) Antibody Pair Kit (with Standard)
MBS2101506-5x96TestsMBS2090685AbPairsSupportPack15x96Tests	MyBiosource	5x96Tests+MBS2090685(AbPairsSupportPack1,5x96Tests)	EUR 1035

Reference 2 Pack 3 100-1000uL; 0.5-5mL; 1-10mL pipettes
E4920000920	Scientific Laboratory Supplies	EACH	EUR 835.62

Reference 2 Pack 1 0.5-10; 10-100; 100-1000uL pipettes - EACH
E4924000908	Scientific Laboratory Supplies	EACH	EUR 1057.05

Reference 2 Pack 2 2-20; 20-200; 100-1000uL pipettes - EACH
E4924000916	Scientific Laboratory Supplies	EACH	EUR 1057.05

Pipetman M Electronic Charging Carousel for upto 5 Pipettes - EACH
FB1001	Scientific Laboratory Supplies	EACH	EUR 634.5

SureStand™ Pipette Stand for 3 pipettes, up to one multi-channel, acrylic
P4403	MTC Bio	1/pack	EUR 55.2
Description: SureStand Pipette Stand for 3 pipettes up to one multi-channel

SureStand™ Pipette Stand for 5 pipettes, up to two multi-channels, acrylic
P4405	MTC Bio	1/pack	EUR 86.4
Description: SureStand Pipette Stand for 5 pipettes up to two multi-channels

SureStand™ Pipette Stand for 8 pipettes, up to six multi-channels, acrylic
P4408	MTC Bio	1/pack	EUR 106.8
Description: SureStand Pipette Stand for 8 pipettes up to six multi-channels

3 ml Graduated Transfer Pipettes, Individually Wrapped, Sterile
B912	ABM	Case of 2000	EUR 225

SureStand™ Pipette Stand for 6 pipettes, up to four multi-channels, acrylic
P4406	MTC Bio	1/pack	EUR 94.8
Description: SureStand Pipette Stand for 6 pipettes up to four multi-channels

Here, we offer an in depth protocol describing learn how to manufacture and calibrate the micropipettes, in addition to learn how to efficiently design, carry out, and troubleshoot MFS experiments. We exemplify our method using the mannequin nematode Caenorhabditis elegans, however by following this protocol, all kinds of dwelling samples, ranging from single cells to multicellular aggregates and millimeter-sized organisms, will be studied in vivo, with a pressure decision as little as 10 pN. A talented (underneath)graduate scholar can grasp the method in ~1-2 months. The entire protocol takes ~1-2 d to complete.